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ea0084op-04-21 | Oral Session 4: Basic 1 | ETA2022

CRYO-electron microscopy structures of human thyroid peroxidase (TPO) in complex with tpo antibodies

Baker Stuart , Nunez Miguel Ricardo , Thomas Daniel , Powell Michael , Furmaniak Jadwiga , Rees Smith Bernard

Objectives: Thyroid peroxidase (TPO) is a key enzyme in the synthesis of thyroid hormones and is a target for autoimmune responses in autoimmune thyroid disease. TPO autoantibody (TPOAb) binding epitopes have been mapped on the peroxidase domain (POD) and the complement control protein like domain (CCP). This study aimed to solve the molecular structures of TPO bound to TPO antibodies.Methods: An extracellular domain (ECD) of human TPO (amino acids; aa 1...

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ea0084op-01-01 | Oral Session 1: Topic Highlights | ETA2022

Cryo-electron microscopy structure of full length TSH receptor in complex with TSH receptor blocking human monoclonal autoantibody K1-70TM

Nunez Miguel Ricardo , Sanders Paul , Allen Lloyd , Evans Michele , Holly Matthew , Johnson William , Sullivan Andrew , Miller-Gallacher Jennifer , Sanders Jane , Furmaniak Jadwiga , Rees Smith Bernard

Objectives: The crystal structures of the TSH receptor (TSHR) leucine rich repeat domain (LRD) bound to TSHR stimulating monoclonal autoantibody M22TM or to TSHR blocking monoclonal autoantibody K1-70TM and antibody free have been solved previously. Cryo-electron microscopy (cryo-EM) was now used to solve the structure of full length TSHR in complex with K1-70TM.Methods: Recombinant human TSHR expressed in CHO cells was i...

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Endocrine Abstracts

CRYO-electron microscopy structures of human thyroid peroxidase (TPO) in complex with tpo antibodies

Cryo-electron microscopy structure of full length TSH receptor in complex with TSH receptor blocking human monoclonal autoantibody K1-70TM

BiosciAbstracts